Peer Review Report for 10.1105/tpc.17.00047 Spatiotemporal Monitoring of Pseudomonas Effectors via Type III Secretion using Split Fluorescent Protein Fragments
نویسندگان
چکیده
Review timeline: TPC2017-00047-BRR Submission received: Jan. 16, 2017 1st Decision: Feb. 21, 2017 revision requested TPC2017-00047-BRR1 1st Revision received: April 24, 2017 2nd Decision: May 20, 2017 revision requested TPC2017-00047-BRR2 2nd Revision received: May 27, 2017 3rd Decision: June 7, 2017 acceptance pending, sent to science editor Final acceptance: June 14, 2017 Advance publication: June 14, 2017
منابع مشابه
Spatiotemporal Monitoring of Pseudomonas syringae Effectors via Type III Secretion Using Split Fluorescent Protein Fragments.
Pathogenic gram-negative bacteria cause serious diseases in animals and plants. These bacterial pathogens use the type III secretion system (T3SS) to deliver effector proteins into host cells; these effectors then localize to different subcellular compartments to attenuate immune responses by altering biological processes of the host cells. The fluorescent protein (FP)-based approach to monitor...
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Pathogenic gram-negative bacteria cause serious diseases in animals and plants. These bacterial pathogens use the type III secretion system (T3SS) to deliver effector proteins into host cells; these effectors then localize to different subcellular compartments to attenuate immune responses by altering biological processes of the host cells. The fluorescent protein (FP)-based approach to monitor...
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Bacterial pathogens use the Type III secretion system to deliver dozens of effector proteins into host cells; for example, the plant pathogen Pseudomonas syringae can deliver more than 30 effectors. These effectors have multiple, nefarious functions that help the pathogen thrive in its host (reviewed in Toraño et al., 2016). Effectors target conserved host processes including pathways for entry...
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To cause disease, diverse pathogens deliver effector proteins into host cells. Pathogen effectors can inhibit defense responses, alter host physiology, and represent important cellular probes to investigate plant biology. However, effector function and localization have primarily been investigated after overexpression in planta. Visualizing effector delivery during infection is challenging due ...
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